| 6 Nov 2019 |
| 23:16:34 |  paniscus | Well it looks very impressive. All that plastic goes in. Remind me what comes out? Forgotten already, clearly not a coffee cup, and plastic recycled to pencils is so 80s |
| 7 Nov 2019 |
| 08:45:46 |  ajp | Q7GXDCTE this was the workshop I was talking about ^^ |
| 13:57:26 |  davidclaytonsgs | Does anyone know if we have filter syringes in the lab? |
| 19:18:51 | |  brigittekock joined the room. |
| 19:18:58 | brigittekock | Hi everyone 🙂 I'm new to the biolab. For my masters at CSM I'll be working on growing keratin through bacterial expression. (the carapace of E.imbricata, hopefully, to be more specific ). Nice to meet you and hopefully see you guys around in the lab 🙂 |
| 19:19:22 | brigittekock | 06G73G65 Cheers, I'll see if I can come 🙂 |
| 8 Nov 2019 |
| 14:15:07 | |  arleen-mm joined the room. |
| 10 Nov 2019 |
| 18:10:33 | davidclaytonsgs | @room Hi guys, I need to make some modifications to the list of things I will finally need for my experiment. For now I would like to purchase the aparamycin (£35.20) from sigma, gel extraction and cleanup kit from NEB (£156). Therefore the total is now £191.2. I have held off form buying my previous list of items because after reviewing it, we don’t need ampicillin for now and I forgot to add the kits to that list I made. These kits are being bought from NEB and will be covered by the £5000 credit NEB gave us when we opened the account.
The gel extraction and cleanup kits are essential for purifying DNA required for downstream reactions and would be very handy for the lab and we do not have any at the moment. I have linked the gel extraction and cleanup kit for anyone to see and have the detailed protocols to use them as well if you would like to look. These kits are being bought from NEB and will be covered by the credit limit the biolab’s NEB account.
Thank you for reading.
https://www.neb.uk.com/products/neb-catalogue/nucleic-acid-purification/monarch-reg;-dna-gel-extraction-kit
https://www.neb.uk.com/products/neb-catalogue/nucleic-acid-purification/monarch-reg;-pcr-amp;-dna-cleanup-kit-(5-mu;g) |
| 18:15:03 | paniscus | Presumably the £5000 is commercial credit, ie the highest amount we can have outstanding on invoices while we arrange payment? |
| 18:15:47 | davidclaytonsgs | yes that is what I assume as well |
| 18:15:50 | davidclaytonsgs | one second |
| 22:32:23 | ajp | Yeah we'd never use that facility. We should ask them to remove it. |
| 11 Nov 2019 |
| 20:29:40 | paniscus | As plastic recycling is a hot topic right now: https://www.theguardian.com/environment/2019/nov/10/research-labs-plastic-waste?CMP=Share_iOSApp_Other |
| 13 Nov 2019 |
| 00:53:02 | |  joey joined the room. |
| 16:52:40 |  clauajanpi | https://www.google.com/amp/s/amp.theatlantic.com/amp/article/553511/ |
| 14 Nov 2019 |
| 23:45:00 | |  guillermo joined the room. |
| 18 Nov 2019 |
| 00:07:10 | |  fred joined the room. |
| 10:31:19 |  francois | well, that was obviously a bad idea.. never inject yourself something with unknown side effects ! |
| 10:31:45 | francois | i guess it is a quicker way to get cancer than smoking |
| 12:03:14 | clauajanpi | lool yes hahaha |
| 19:35:51 |  j.oldham | Hi! I am looking to grow some vibrio harveyi and do a few experiments related to quorum sensing.
06G73G65 I was speaking to you about it a few weeks ago if you recall. I have pulled out a few sources with info on quorum sensing and growing vibrio harveyi below (I was initially looking at using vibrio fischeri but changed for various reasons). Do you think this will be feasible/ an interesting project? I could come up to the space at some point to perhaps discuss the details some more.
Here is some general info on quorum sensing for anyone who is interested!..
• Ted talk by Bonnie Bassler, who started studying quorum sensing when it was first discovered in the 70’s and since then has produced a lot of subsequent research and discoveries: https://www.ted.com/talks/bonnie_bassler_on_how_bacteria_communicate/transcript?language=en
• There are a lot of reviews on quorum sensing, this one is pretty good and goes into detail about vibrio harveyi which is what I am looking to grow: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4313539/
For growing vibrio harveyi:
• Vibrio Harveyi wild type can be bought hear (actually named v.campbelli but it’s essentially the same thing apparently). Price tag is a bit of a low blow, not sure if anyone is aware of cheaper sources… https://www.lgcstandards-atcc.org/products/all/BAA-1116.aspx
• The medium is ATCC Medium: 2746 Autoinducer Bioassay (AB) Medium. https://www.lgcstandards-atcc.org/~/media/F0C5AC9D96C345049F8AA78CCC2AB46A.ashx
• I found the above ATCC references from this paper, it provides a bit of info about creating the medium although the one they use is slightly different https://iubmb.onlinelibrary.wiley.com/doi/full/10.1002/bmb.20262 |
| 22:38:40 | |  sara joined the room. |
| 19 Nov 2019 |
| 17:30:09 | clauajanpi | is anyone with lab access going to be here today ? |
| 17:31:01 | clauajanpi | I need to check the max temp of the freezer. That big one |
| 18:11:54 |  darigovresearch | I will be in but at roughly 8 ish |
| 20:15:05 | |  julie joined the room. |
| 22:44:22 | ajp | It's -70oC KDU171QD though you can probably treat it as -80oC perfectly fine |
| 22:47:35 | clauajanpi | yes no worries, sorted out. For the moment we only need to buy ampicillin |
| 20 Nov 2019 |
| 22:38:12 | |  josef joined the room. |
| 21 Nov 2019 |
| 08:47:35 | |  vroni joined the room. |
