| 27 Feb 2021 |
|  @raf:matrix.org left the room. | 13:52:36 |
 parichehr mehrafshar (Telegram) | * hi every one
I like to learn bioinformation and biopython but I don't know how to start.
could you please give me some advices? | 13:52:40 |
 SHAAG (Telegram) | In reply to parichehr mehrafshar (Telegram)
hi every one
I like to learn bioinformation and biopython but I don't know how to start.
could you please give me some advices? Youtube is the best place to start learning | 14:04:46 |
| 1 Mar 2021 |
|  Nani Munni (Telegram) joined the room. | 06:17:31 |
|  purpleark joined the room. | 16:07:33 |
| 3 Mar 2021 |
|  N m (Telegram) joined the room. | 20:25:07 |
| 4 Mar 2021 |
|  Zhanerke🍀 (Telegram) changed their display name from Zhan (Telegram) to Zhanerke (Telegram). | 19:54:26 |
| Zhanerke🍀 (Telegram) changed their profile picture. | 19:54:27 |
| 6 Mar 2021 |
|  PR Sinha (Telegram) changed their display name from Priyansha (Telegram) to Prs (Telegram). | 17:10:25 |
| 11 Mar 2021 |
 rebelCoder (Telegram) | Hey! So it looks like the code does not generate the top (longest) sequence but generates the second one, right? | 17:29:57 |
| rebelCoder (Telegram) | Yeah. Looks like (from your images) all sequences are generated correctly but it is missing the first one. We can try debugging the code to see what is happening at each step of our algorithm (as good way to l;earn programming) and try figuring out why it misses that first sequence. | 17:31:32 |
| rebelCoder (Telegram) | Billa can you please provide the DNA sequence you are using? | 17:31:55 |
| rebelCoder (Telegram) | * Yeah. Looks like (from your images) all sequences are generated correctly but it is missing the first one. We can try debugging the code to see what is happening at each step of our algorithm (it is a good way to learn programming) and try figuring out why it misses that first sequence. | 17:32:36 |
| 15 Mar 2021 |
|  Billa Bong (Telegram) joined the room. | 15:49:49 |
| Billa Bong (Telegram) | thanks for helping! | 15:49:57 |
| rebelCoder (Telegram) | In reply to Billa Bong (Telegram)
@rebelCoder Hi there! Sorry for the late respons, i dont use Telegram often, so i didn't see it :) I'm using this sequence: https://www.ncbi.nlm.nih.gov/nuccore/JF923567.1 Cool! Thanks! I will look at this sequence to see why our code misses it. Thanks for your input. | 18:04:42 |
| Billa Bong (Telegram) | Great! and of course. Thank you :) | 18:08:14 |
| 16 Mar 2021 |
|  Aditya Raj (Telegram) joined the room. | 19:04:00 |
 Bioinfo (Telegram) | @query#BigScape#cytoscape, can anybody help me importing BigScape data into cytoscape | 20:08:34 |
| 19 Mar 2021 |
|  Carlo (Telegram) changed their display name from Carlo Fortunato (Telegram) to Carlo (Telegram). | 22:51:27 |
| 20 Mar 2021 |
|  Akash Khatri (Telegram) joined the room. | 19:44:45 |
| 21 Mar 2021 |
| PR Sinha (Telegram) | On the pdb site, how the residue count is more than sequence length? | 09:58:17 |
| PR Sinha (Telegram) | In reply to Prs (Telegram)
On the pdb site, how the residue count is more than sequence length? For any protein | 10:03:12 |
| Carlo (Telegram) | In reply to Prs (Telegram)
On the pdb site, how the residue count is more than sequence length? Maybe that protein has been modified for structure solving 🤔 you mean: for each chain there are additional residues compared to the sequence you find on uniprotKB? | 10:14:56 |
| Carlo (Telegram) | It is probably due to the cloning process: when you clone your sequence inside a plasmid, it adds residues and changes the starting codon (the most common case is the addition of Histidines tail at N-end, but also at C-end or both... It is useful for the protein purification) | 10:18:38 |
| Carlo (Telegram) | It could be also due to a stabilization matter (maybe they found that the native protein didn't give crystals for X-Rays crystallography and authors had to modify it, add pieces, and so on) | 10:21:10 |
| Carlo (Telegram) | Usually these kind of mutations are specified | 10:21:42 |
| PR Sinha (Telegram) | But I couldn't find any such info mentioned there | 10:57:58 |
| PR Sinha (Telegram) | For eg. Check 6F1E - interferon gamma on pdb | 10:58:44 |
| PR Sinha (Telegram) | There u can see the residue count of 270 but seq length 175 | 10:59:13 |
