21 Mar 2021 |
Carlo (Telegram) | Ok I am going to check it | 11:43:33 |
Carlo (Telegram) | 270 is the sum of the number of residues present in the pdb structure (both chains) | 11:58:38 |
Carlo (Telegram) | Download image.jpeg | 11:59:44 |
Carlo (Telegram) | In red boxes you can see the residues missing in the crystallographic structure. | 11:59:44 |
Carlo (Telegram) | The length of each one (including red boxes) is 175 (look at the numberings above the sequences) | 12:00:40 |
Carlo (Telegram) | If you subtract to each one the red boxes you get 143 for chain A and 127 for chain B, and 143+127=270 | 12:02:50 |
PR Sinha (Telegram) | In reply to Carlo (Telegram) In red boxes you can see the residues missing in the crystallographic structure. Ohh..I get it now | 12:05:35 |
PR Sinha (Telegram) | Thanks a lot | 12:05:54 |
Carlo (Telegram) | In reply to Carlo (Telegram) In red boxes you can see the residues missing in the crystallographic structure. Pay attention now to the numberings aside: they tells you the position of a specific aminoacid in the original sequence... As you can see in chain A the first available residue in the structure is the 25th (an H). In the chain B the first is the 28th (an A) | 12:06:16 |
PR Sinha (Telegram) | In reply to Carlo (Telegram) Pay attention now to the numberings aside: they tells you the position of a specific aminoacid in the original sequence... As you can see in chain A the first available residue in the structure is the 25th (an H). In the chain B the first is the 28th (an A) Yes | 12:07:06 |
Carlo (Telegram) | This numeration is probably referred to the protein sequence expected from the cloning...and it is probably same or similar in the uniprotKB...☺️ | 12:07:23 |
Carlo (Telegram) | You're welcome | 12:07:28 |
PR Sinha (Telegram) | So in any case, total length will be equal to or greater than residue count, right? | 12:10:32 |
PR Sinha (Telegram) | In reply to Carlo (Telegram) This numeration is probably referred to the protein sequence expected from the cloning...and it is probably same or similar in the uniprotKB...☺️ U mean seq length (here 175) ? | 12:12:59 |
Carlo (Telegram) | Download image.jpeg | 12:17:22 |
Carlo (Telegram) | The missing serine is the 24th residue in the original length... Was just saying this | 12:17:22 |
PR Sinha (Telegram) | In reply to Carlo (Telegram) The missing serine is the 24th residue in the original length... Was just saying this Ok | 12:18:20 |
Carlo (Telegram) | And it's the 1st in the structure (sincerely don't know why it counts if it misses also in the pdb...🙌) | 12:18:43 |
Carlo (Telegram) | Just to warn you if you want to align it with the original one, they crystallized (or find the electron density map) for only a part of the protein | 12:20:00 |
PR Sinha (Telegram) | In reply to Carlo (Telegram) Just to warn you if you want to align it with the original one, they crystallized (or find the electron density map) for only a part of the protein Okay, sure | 12:48:07 |
PR Sinha (Telegram) | In reply to Carlo (Telegram) In red boxes you can see the residues missing in the crystallographic structure. Are u visualizing the files on chimera? | 12:57:53 |
Carlo (Telegram) | Yep | 12:58:13 |
PR Sinha (Telegram) | Okk | 12:59:44 |
24 Mar 2021 |
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25 Mar 2021 |
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30 Mar 2021 |
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31 Mar 2021 |
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